Article
A one-step real-time RT-PCR helps to identify mixed rotavirus infections in Mexico.
Diagnostic microbiology and infectious disease - 1 Dec 2018
Anaya-Molina Yazmin, De La Cruz Hernández Sergio Isaac, Andrés-Dionicio Atenea Estela, Terán-Vega Heidi Lizbeth, Méndez-Pérez Héctor, Castro-Escarpulli Graciela, García-Lozano Herlinda
Abstract excerpt
Rotaviruses continue being the most important pathogens responsible of diarrhea in young children worldwide. Seminested reverse transcription polymerase chain reaction (RT-PCR) is used to determine rotavirus genotype; however, this technique employs multistep procedures. The real-time RT-PCR is a fast and reliable tool that can be used as rotavirus genotyping tool, especially in rotavirus outbreaks. In this...
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