Article
Genotyping based on thermal denaturation of amplification products identifies species of the Mycobacterium tuberculosis complex.
Journal of medical microbiology - 1 Sept 2018
Domínguez-Zepahua Mariel, Hernández-Arteaga Socorro, López-Revilla Rubén
Abstract excerpt
PURPOSE: To develop a fast and inexpensive genotyping assay to identify the Mycobacterium tuberculosis complex (MTC) species most prevalent in human tuberculosis (TB), based on the thermal denaturation profiles of PCR products from mycobacterial 16S rDNA and three MTC genomic regions of difference (RD). METHODOLOGY: Genotypes were determined by the presence and thermal denaturation profiles of the amplicons...
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