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Article

Improved phosphoproteomic analysis for phosphosignaling and active-kinome profiling in Matrigel-embedded spheroids and patient-derived organoids.

Scientific reports - 30 Jul 2018

Abe Yuichi, Tada Asa, Isoyama Junko, Nagayama Satoshi, Yao Ryoji, Adachi Jun, Tomonaga Takeshi

Abstract excerpt

Many attempts have been made to reproduce the three-dimensional (3D) cancer behavior. For that purpose, Matrigel, an extracellular matrix from Engelbreth-Holm-Swarm mouse sarcoma cell, is widely used in 3D cancer models such as scaffold-based spheroids and patient-derived organoids. However, severe ion suppression caused by contaminants from Matrigel hampers large-scale phosphoproteomics. In the present study, we...

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PMID: 30061712
DOI: 10.1038/s41598-018-29837-1
PMCID: PMC6065387

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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