Article
Relocation of the plastid rbcL gene to the nucleus yields functional ribulose-1,5-bisphosphate carboxylase in tobacco chloroplasts.
Proceedings of the National Academy of Sciences of the United States of America - 1 Mar 1994
Kanevski I, Maliga P
Abstract excerpt
The conserved plastid localization of rbcL suggests that biosynthesis of the large subunit of ribulose-1,5-bisphosphate carboxylase [Rubisco; 3-phospho-D-glycerate carboxy-lyase (dimerizing), EC 4.1.1.39] in chloroplasts is required to obtain functional enzyme. To examine the validity of this hypothesis, we relocated the plastid rbcL gene to the nucleus. First, we deleted the rbcL gene from the tobacco plastid...
Topics
- Base Sequence
- Cell Nucleus
- Chloroplasts
- DNA
- Drug Resistance
- Gene Deletion
- Genes, Plant
- Genetic Complementation Test
- Molecular Sequence Data
- Phenotype
- Plants, Genetically Modified