Article
Viral Entry Properties Required for Fitness in Humans Are Lost through Rapid Genomic Change during Viral Isolation.
mBio - 3 Jul 2018
Iketani Sho, Shean Ryan C, Ferren Marion, Makhsous Negar, Aquino Dolly B, des Georges Amedee, Rima Bert, Mathieu Cyrille, Porotto Matteo, Moscona Anne, Greninger Alexander L
Abstract excerpt
Human parainfluenza viruses cause a large burden of human respiratory illness. While much research relies upon viruses grown in cultured immortalized cells, human parainfluenza virus 3 (HPIV-3) evolves in culture. Cultured viruses differ in their properties compared to clinical strains. We present a genome-wide survey of HPIV-3 adaptations to culture using metagenomic next-generation sequencing of matched pairs...
Topics
- Adaptation, Biological
- DNA Mutational Analysis
- Genetic Fitness
- Genome, Viral
- Humans
- Mutation
- Parainfluenza Virus 3, Human
- Respirovirus Infections
- Serial Passage
- Virus Cultivation