Article
[Construction and characterization of an hcp mutant of swine Bordetella bronchiseptica].
Wei sheng wu xue bao = Acta microbiologica Sinica - 4 Feb 2017
Chang Yuhui, Zhang Hongfeng, Peng Zhong, Li Hui, Chen Huanchun, Wu Bin
Abstract excerpt
Objective: We constructed Bordetella bronchiseptica QH0814 hcp mutant to characterize its pathogenicity.[Methods] Through the homologous recombination mediated by a suicide plasmid pRE112, we acquired the mutant QH0814Δhcp successfully. Then, we evaluated the growth condition, the ability of adhesion and invasion, the median lethal dose (LD50) and the infection capacity. Methods: Through the homologous...
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