Article
Flow Cytometry Data Preparation Guidelines for Improved Automated Phenotypic Analysis.
Journal of immunology (Baltimore, Md. : 1950) - 15 May 2018
Jimenez-Carretero Daniel, Ligos José M, Martínez-López María, Sancho David, Montoya María C
Abstract excerpt
Advances in flow cytometry (FCM) increasingly demand adoption of computational analysis tools to tackle the ever-growing data dimensionality. In this study, we tested different data input modes to evaluate how cytometry acquisition configuration and data compensation procedures affect the performance of unsupervised phenotyping tools. An analysis workflow was set up and tested for the detection of changes in...
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