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Article

OMIP-118: A 38-Marker Spectral Flow Cytometry Panel to Assess Human Regulatory T Cell Phenotype and Lineage Plasticity.

Cytometry. Part A : the journal of the International Society for Analytical Cytology - 1 Oct 2025

Bhattacharya Nilika, Jugler Collin, Hill Jessica C, Copple Kade R, Nikhat Sameena, Khosravi-Maharlooei Mohsen, Ager Casey R

Abstract excerpt

We report the optimization of a 38-parameter spectral flow cytometry panel to identify, phenotype, and assess lineage plasticity of human regulatory T cells (Tregs). Tregs are an indispensable T cell lineage with pleiotropic tolerogenic functions whose activities contribute critically to numerous disease settings including cancer, autoimmunity, and infectious disease, among others. Phenotypic and functional...

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PMID: 41047584
DOI: 10.1002/cyto.a.24966

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