Article
Standardized high-dimensional spectral cytometry protocol and panels for whole blood immune phenotyping in clinical and translational studies.
Cytometry. Part A : the journal of the International Society for Analytical Cytology - 1 Feb 2024
Dott Tom, Culina Slobodan, Chemali Rene, Mansour Cedric Ait, Dubois Florian, Jagla Bernd, Doisne Jean Marc, Rogge Lars, Huetz François, Jönsson Friederike, Commere Pierre-Henri, Di Santo James, Terrier Benjamin, Quintana-Murci Lluis, Duffy Darragh, Hasan Milena
Abstract excerpt
Flow cytometry is the method of choice for immunophenotyping in the context of clinical, translational, and systems immunology studies. Among the latter, the Milieu Intérieur (MI) project aims at defining the boundaries of a healthy immune response to identify determinants of immune response variation. MI used immunophenotyping of a 1000 healthy donor cohort by flow cytometry as a principal outcome for immune...
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