Article
Droplet Digital PCR-Based Chimerism Analysis for Primary Immunodeficiency Diseases.
Journal of clinical immunology - 1 Apr 2018
Okano Tsubasa, Tsujita Yuki, Kanegane Hirokazu, Mitsui-Sekinaka Kanako, Tanita Kay, Miyamoto Satoshi, Yeh Tzu-Wen, Yamashita Motoi, Terada Naomi, Ogura Yumi, Takagi Masatoshi, Imai Kohsuke, Nonoyama Shigeaki, Morio Tomohiro
Abstract excerpt
OBJECTIVE: In the current study, we aimed to accurately evaluate donor/recipient or male/female chimerism in samples from patients who underwent hematopoietic stem cell transplantation (HSCT). METHODS: We designed the droplet digital polymerase chain reaction (ddPCR) for SRY and RPP30 to detect the male/female chimerism. We also developed mutation-specific ddPCR for four primary immunodeficiency diseases....
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