Article
Single-Nucleotide Polymorphism Genotyping of exoS in Pseudomonas aeruginosa Using Dual-Color Fluorescence Hybridization and Magnetic Separation.
Journal of biomedical nanotechnology - 1 Jan 2018
Tang Yongjun, Ali Zeeshan, Dai Jianguo, Liu Xiaolong, Wu Yanqi, Chen Zhu, He Nongyue, Li Song, Wang Lijun
Abstract excerpt
Pseudomonas aeruginosa exoS gene contains important replacement (non-synonymous) single-nucleotide polymorphism (SNP) loci, of which mutations in loci 162 (G162A) and 434 (G434C) in exoS greatly affects virulence. The present study aimed to develop an SNP-based classification method for exoS loci (G162A and G434C), using magnetic enrichment polymerase chain reaction, magnetic separation, and dual-color...
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