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Article

Single-nucleotide-polymorphism mapping of the Pseudomonas aeruginosa type III secretion toxins for development of a diagnostic multiplex PCR system.

Journal of clinical microbiology - 1 Aug 2003

Ajayi Temitayo, Allmond Leonard R, Sawa Teiji, Wiener-Kronish Jeanine P

Abstract excerpt

We mapped the coding single nucleotide polymorphisms in four toxin genes-exoS, exoT, exoU, and exoY-of the Pseudomonas aeruginosa type III secretion system among several clinical isolates. We then used this information to design a multiplex PCR assay based on the simultaneous amplification of fragments of these genes. Eight strains of known genotype were used to test our multiplex PCR method, which showed 100%...

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Identifiers

PMID: 12904350
DOI: 10.1128/JCM.41.8.3526-3531.2003
PMCID: PMC179785

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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