Article
Mutational Analysis of Binding Protein-Chemoreceptor Interactions.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2018
Seely Andrew L
Abstract excerpt
Interactions between ligands and chemoreceptors in Escherichia coli and Salmonella enterica can be studied through genetic manipulation of the actors involved. Sequence analysis and modeling can reveal potential sites of interaction, and these sites can be deleted or mutated and the effects tested through various in vivo chemotaxis assays to ascertain their importance during interaction. Here, the approach for...
Topics
- Amino Acid Sequence
- Binding Sites
- Carrier Proteins
- Chemotaxis
- Escherichia coli
- Escherichia coli Proteins
- Ligands
- Methyl-Accepting Chemotaxis Proteins
- Mutation
- Protein Binding
