Article
Stabilization of mesophilic Allochromatium vinosum cytochrome c' through specific mutations modeled by a thermophilic homologue.
Bioscience, biotechnology, and biochemistry - 1 Feb 2018
Yamane-Koshizawa Daisuke, Fujii Sotaro, Maruno Takahiro, Kobayashi Yuji, Yamanaka Masaru, Wakai Satoshi, Sambongi Yoshihiro
Abstract excerpt
AVCP cytochrome c' from mesophilic Allochromatium vinosum exhibits lower stability than a thermophilic counterpart, Hydrogenophilus thermoluteolus cytochrome c' (PHCP), in which the six specific amino acid residues that are not conserved in AVCP are responsible for its stability. Here we measured the stability of AVCP variants carrying these specific residues instead of the original AVCP ones. Among the six...
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