Article
Comparative study on stabilization mechanism of monomeric cytochrome c5 from deep-sea piezophilic Shewanella violacea.
Bioscience, biotechnology, and biochemistry - 1 Dec 2016
Masanari Misa, Fujii Sotaro, Kawahara Kazuki, Oki Hiroya, Tsujino Hirofumi, Maruno Takahiro, Kobayashi Yuji, Ohkubo Tadayasu, Wakai Satoshi, Sambongi Yoshihiro
Abstract excerpt
Monomeric cytochrome c5 from deep-sea piezophilic Shewanella violacea (SVcytc5) was stable against heat and denaturant compared with the homologous protein from shallow-sea piezo-sensitive Shewanella livingstonensis (SLcytc5). Here, the SVcytc5 crystal structure revealed that the Lys-50 side chain on the flexible loop formed a hydrogen bond with heme whereas that of corresponding hydrophobic Leu-50 could not form...
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