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Virus Binding and Internalization Assay for Adeno-associated Virus

1 Jan 2017

Abstract excerpt

The binding and internalization of adeno-associated virus (AAV) is an important determinant of viral infectivity and tropism. The ability to dissect these two tightly connected cellular processes would allow better understanding and provide insight on virus entry and trafficking. In the following protocol, we describe a quantitative PCR (qPCR) based method to determine the amount of vector bound to the cell...

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PMID: 29226179
DOI: 10.21769/bioprotoc.2110

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