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Article

Initial germ cell to somatic cell ratio impacts the efficiency of SSC expansion in vitro.

Systems biology in reproductive medicine - 1 Feb 2018

Gat Itai, Maghen Leila, Filice Melissa, Kenigsberg Shlomit, Wyse Brandon, Zohni Khaled, Saraz Peter, Fisher Andrée Gauthier, Librach Clifford

Abstract excerpt

Spermatogonial Stem Cell (SSC) expansion in vitro remains a major challenge in efforts to preserve fertility among pubertal cancer survivor boys. The current study focused on innovative approaches to optimize SSC expansion. Six- to eight-week-old CD-1 murine testicular samples were harvested by mechanical and enzymatic digestion. Cell suspensions were incubated for differential plating (DP). After DP, we...

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Identifiers

PMID: 29193985
DOI: 10.1080/19396368.2017.1406013

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UTF1

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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