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Optimal culture conditions are critical for efficient expansion of human testicular somatic and germ cells in vitro.

Fertility and sterility - 1 Mar 2017

Gat Itai, Maghen Leila, Filice Melissa, Wyse Brandon, Zohni Khaled, Jarvi Keith, Lo Kirk C, Gauthier Fisher Andrée, Librach Clifford

Abstract excerpt

OBJECTIVE: To optimize culture conditions for human testicular somatic cells (TSCs) and spermatogonial stem cells. DESIGN: Basic science study. SETTING: Urology clinic and stem cell research laboratory. PATIENT(S): Eight human testicular samples. INTERVENTIONS(S): Testicular tissues were processed by mechanical and enzymatic digestion. Cell suspensions were subjected to differential plating (DP) after which...

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Identifiers

PMID: 28259258
DOI: 10.1016/j.fertnstert.2016.12.028

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