The N253F mutant structure of trehalose synthase from Deinococcus radiodurans reveals an open active-site topology.

Abstract excerpt

Trehalose synthase (TS) catalyzes the reversible conversion of maltose to trehalose and belongs to glycoside hydrolase family 13 (GH13). Previous mechanistic analysis suggested a rate-limiting protein conformational change, which is probably the opening and closing of the active site. Consistently, crystal structures of Deinococcus radiodurans TS (DrTS) in complex with the inhibitor Tris displayed an enclosed...

Topics

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• Catalytic Domain
• Crystallography, X-Ray
• Deinococcus
• Glucosyltransferases
• Models, Molecular