Article
Harnessing a catalytic lysine residue for the one-step preparation of homogeneous antibody-drug conjugates.
Nature communications - 24 Oct 2017
Nanna Alex R, Li Xiuling, Walseng Even, Pedzisa Lee, Goydel Rebecca S, Hymel David, Burke Terrence R, Roush William R, Rader Christoph
Abstract excerpt
Current strategies to produce homogeneous antibody-drug conjugates (ADCs) rely on mutations or inefficient conjugation chemistries. Here we present a strategy to produce site-specific ADCs using a highly reactive natural buried lysine embedded in a dual variable domain (DVD) format. This approach is mutation free and drug conjugation proceeds rapidly at neutral pH in a single step without removing any charges....
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