Article
Amino acid residues critical for DNA binding and inducer recognition in CbnR, a LysR-type transcriptional regulator from Cupriavidus necator NH9.
Bioscience, biotechnology, and biochemistry - 1 Nov 2017
Moriuchi Ryota, Takada Kaori, Takabayashi Masae, Yamamoto Yuko, Shimodaira Jun, Kuroda Naoko, Akiyama Emiko, Udagawa Mayumi, Minai Ryoichi, Fukuda Masao, Senda Toshiya, Ogawa Naoto
Abstract excerpt
CbnR, a LysR-type transcriptional regulator from Cupriavidus necator NH9, activates the transcription of chlorocatechol-degradative enzymes. To activate the transcription, CbnR needs to bind not only to the cbnA promoter but also to the inducer. In this study, the transcriptional activity and DNA-binding activity of twenty-five mutants of CbnR were analyzed. Of the 17 mutants of the DNA-binding domain, 11 mutants...
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