Article
Detection of APOBEC3 Proteins and Catalytic Activity in Urothelial Carcinoma.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2018
Jaguva Vasudevan Ananda Ayyappan, Goering Wolfgang, Häussinger Dieter, Münk Carsten
Abstract excerpt
Members of the APOBEC3 (A3) family of enzymes were shown to act in an oncogenic manner in several cancer types. Immunodetection of APOBEC3A (A3A), APOBEC3B (A3B), and APOBEC3G (A3G) proteins is particularly challenging due to the large sequence homology of these proteins and limited availability of antibodies. Here we combine independent immunoblotting with an in vitro activity assay technique, to detect and...
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