Article
Forward genetic screening of insertional mutants.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2006
Schneider Anja, Leister Dario
Abstract excerpt
Insertional mutagenesis has contributed to the success of forward genetics in Arabidopsis thaliana. The availability of large collections of lines mutagenized by either transposon or T-DNA insertions, in combination with the systematic sequencing of insertion/genome junctions, enables the identification of mutations for any given gene in the nuclear genome of this plant species. Protocols for the identification...
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