Article
[Nuclease activity of the recombinant plancitoxin-1-like proteins with mutations in the active site from Trichinella spiralis].
Sheng wu gong cheng xue bao = Chinese journal of biotechnology - 25 Aug 2017
Liao Chengshui, Wang Xiaoli, Tian Wenjing, Zhang Mengke, Zhang Chunjie, Li Yinju, Wu Tingcai, Cheng Xiangchao
Abstract excerpt
Although there are 125 predicted DNase Ⅱ-like family genes in the Trichinella spiralis genome, plancitoxin-1-like (Ts-Pt) contains the HKD motif, a typical conserved region of DNase Ⅱ, in N- and C-terminal. It is generally believed that histidine is the active site in DNase Ⅱ. To study the nuclease activity of recombinant Ts-Pt with mutations in the active site from T. spiralis, different fragments of the mutated...
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