Article
[Cloning and characterization of p43 cDNA from Trichinella spiralis].
Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases - 30 Dec 2005
Guo Heng, Li Lian-rui, Liu Ming-yuan, Wu Xiu-ping, Sun Shu-min, Fu Bao-quan, Gao Chang-ling, Lu Qiang, Chen Qi-jun, Boireau Pascal
Abstract excerpt
OBJECTIVE: To clone and characterize the p43 cDNA from muscle larvae cDNA library of Trichinella spiralis (Ts) Chinese isolate. METHODS: PCR technique was used to amplify the target cDNA from muscle larvae cDNA library. After cloned in pMD18T vector, it was transformed into E. coli NovaBlue. The positive clones were sequenced and the cDNA was cloned into pET28a expression vector. After induced by IPTG, the...
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