Article
A novel detection procedure for mutations in the 23S rRNA gene of Mycoplasma pneumoniae with peptide nucleic acid-mediated loop-mediated isothermal amplification assay.
Journal of microbiological methods - 1 Oct 2017
Sakai Jun, Maeda Takuya, Tarumoto Norihito, Misawa Kazuhisa, Tamura Shinsuke, Imai Kazuo, Yamaguchi Toshiyuki, Iwata Satoshi, Murakami Takashi, Maesaki Shigefumi
Abstract excerpt
Rapid and easy detection of a single nucleotide point mutation of bacterial genes, which is directly linked to drug susceptibility, is essential for the proper use of antimicrobial agents. Here, we established a detection method using a peptide nucleic acid mediated loop-mediated amplification (LAMP) assay for macrolide (ML)-susceptible Mycoplasma pneumoniae. This assay specifically detected the absence of...
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