Article
Development and evaluation of a loop-mediated isothermal amplification assay for rapid detection of Mycoplasma pneumoniae
28 Sept 2005
Abstract excerpt
A loop-mediated isothermal amplification (LAMP) assay for the rapid detection of Mycoplasma pneumoniae was developed and evaluated. The assay specifically amplified only M. pneumoniae sequences, and no cross-reactivity was observed for other Mycoplasma species or respiratory bacterial species. The detection limit for this assay was found to be 2 x 10(2) copies, corresponding to 2-20 colour changing units of M....
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