Article
Effect of extra cysteine residue of new mutant 1Ax1 subunit on the functional properties of common wheat.
Scientific reports - 8 Aug 2017
Li Miao, Wang Yaqiong, Ma Fengyun, Zeng Jian, Chang Junli, Chen Mingjie, Li Kexiu, Yang Guangxiao, Wang Yuesheng, He Guangyuan
Abstract excerpt
Subunit pair 1Dx5 + 1Dy10 was recognized as superior subunit combination in wheat and contained an extra repetitive-domain cysteine residue in 1Dx5 that was important for understanding the formation of dough viscoelasticity. In this research, one specific serine codon of the 1Ax1 gene corresponding to the extra cysteine residue of 1Dx5 was substituted by a cysteine codon through site-directed mutagenesis. Four...
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