Article
Establishment of highly metastatic KRAS mutant lung cancer cell sublines in long-term three-dimensional low attachment cultures.
PloS one - 1 Jan 2017
Nakano Tomoyuki, Kanai Yoshihiko, Amano Yusuke, Yoshimoto Taichiro, Matsubara Daisuke, Shibano Tomoki, Tamura Tomoko, Oguni Sachiko, Katashiba Shizuka, Ito Takeshi, Murakami Yoshinori, Fukayama Masashi, Murakami Takashi, Endo Shunsuke, Niki Toshiro
Abstract excerpt
Decreased cell-substratum adhesion is crucially involved in metastasis. Previous studies demonstrated that lung cancer with floating cell clusters in histology is more likely to develop metastasis. In the present study, we investigated whether cancer cells in long-term, three-dimensional low attachment cultures acquire high metastatic potential; these cells were then used to examine the mechanisms underlying...
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