Article
Simultaneous Determination of Two Subdomain Folding Rates Using the "Transfer-Quench" Method.
Biophysical journal - 9 May 2017
Rahamim Gil, Amir Dan, Haas Elisha
Abstract excerpt
The investigation of the mechanism of protein folding is complicated by the context dependence of the rates of intramolecular contact formation. Methods based on site-specific labeling and ultrafast spectroscopic detection of fluorescence signals were developed for monitoring the rates of individual subdomain folding transitions in situ, in the context of the whole molecule. However, each site-specific labeling...
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