Article
Neutralization of genotype 2 porcine epidemic diarrhea virus strains by a novel monoclonal antibody.
Virology - 1 Jul 2017
Liu Jianbo, Shi Hongyan, Chen Jianfei, Zhang Xin, Ji Zhaoyang, Yuan Jing, Shi Da, Cao Liyan, Zhu Xiangdong, Dong Hui, Wang Xiaobo, Zhang Jialin, Feng Li
Abstract excerpt
Porcine epidemic diarrhea virus (PEDV) has two genotypes, G1 and G2. To research the immunogenicity differences of PEDV G1 and G2 genotype strains and obtain a neutralizing monoclonal antibody (mAb), we inoculated specific-pathogen-free BALB/c mice with a newly emerged strain, PEDV-LNCT2. After immunizations, cells from the spleen of the mice were fused with SP2/0 myeloma cells. Following culturing and...
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