Article
Assessment of the interlaboratory variability and robustness of JAK2V617F mutation assays: A study involving a consortium of 19 Italian laboratories.
Oncotarget - 16 May 2017
Perricone Margherita, Palandri Francesca, Ottaviani Emanuela, Angelini Mario, Bagli Laura, Bellesia Enrica, Donati Meris, Gemmati Donato, Zucchini Patrizia, Mancini Stefania, Marchica Valentina, Trubini Serena, De Matteis Giovanna, Di Zacomo Silvia, Favarato Mosè, Fioroni Annamaria, Bolzonella Caterina, Maccari Giorgia, Navaglia Filippo, Gatti Daniela, Toffolatti Luisa, Orlandi Linda, Laloux Vèronique, Manfrini Marco, Galieni Piero, Giannini Barbara, Tieghi Alessia, Barulli Sara, Serino Maria Luisa, Maccaferri Monica, Scortechini Anna Rita, Giuliani Nicola, Vallisa Daniele, Bonifacio Massimiliano, Accorsi Patrizia, Salbe Cristina, Fazio Vinicio, Gusella Milena, Toffoletti Eleonora, Salvucci Marzia, Svaldi Mirija, Gherlinzoni Filippo, Cassavia Francesca, Orsini Francesco, Martinelli Giovanni
Abstract excerpt
To date, a plenty of techniques for the detection of JAK2V617F is used over different laboratories, with substantial differences in specificity and sensitivity. Therefore, to provide reliable and comparable results, the standardization of molecular techniques is mandatory.A network of 19 centers was established to 1) evaluate the inter- and intra-laboratory variability in JAK2V617F quantification, 2) identify the...
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