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Article

Genotyping of RHD c.1227G>A allele by melting curve analysis.

Transfusion and apheresis science : official journal of the World Apheresis Association : official journal of the European Society for Haemapheresis - 1 Jun 2021

Wang Ni, Fan Yaxin, Zhou Shihang, Shao Linnan, Xia Yuexin, Liu Ming

Abstract excerpt

BACKGROUND: DEL is the weakest known D-positive phenotype and is detectable only by adsorption and elution tests. RHD c.1227G>A is an important marker for DEL phenotype in East Asians. The aim of this study was to develop a method for RHD c.1227G>A genotyping by single-tube PCR with melting curve analysis. METHODS: Two GC-rich tails of different lengths were attached to the 5'-end of allele-specific primers for...

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PMID: 33642156
DOI: 10.1016/j.transci.2021.103078

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