Article
Rapid discrimination of blaIMP-1, blaIMP-6, and blaIMP-34 using a multiplex PCR.
Journal of microbiological methods - 1 Apr 2017
Kayama Shizuo, Ohge Hiroki, Sugai Motoyuki
Abstract excerpt
Stealth-type carbapenem-resistant Enterobacteriaceae that are resistant to almost all β-lactams except imipenem is emerging in Japan. This resistance is mediated by specific variants of the metallo-β-lactamases (blaIMP-6 or blaIMP-34) that differs by one amino acid from the common variant blaIMP-1. We developed an amplification refractory mutation system (ARMS) PCR assay enabling rapid, sequence independent,...
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