Targeted CRISPR disruption reveals a role for RNase MRP RNA in human preribosomal RNA processing.

Abstract excerpt

MRP RNA is an abundant, essential noncoding RNA whose functions have been proposed in yeast but are incompletely understood in humans. Mutations in the genomic locus for MRP RNA cause pleiotropic human diseases, including cartilage hair hypoplasia (CHH). Here we applied CRISPR-Cas9 genome editing to disrupt the endogenous human MRP RNA locus, thereby attaining what has eluded RNAi and RNase H experiments:...

Topics

• Cell Proliferation
• Clustered Regularly Interspaced Short Palindromic Repeats
• Endoribonucleases
• HeLa Cells
• Humans
• Mutation
• RNA Precursors
• RNA, Small Interfering
• Ribonuclease H