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Article

HPV Genotyping of Modified General Primer-Amplicons Is More Analytically Sensitive and Specific by Sequencing than by Hybridization.

PloS one - 1 Jan 2017

Meisal Roger, Rounge Trine Ballestad, Christiansen Irene Kraus, Eieland Alexander Kirkeby, Worren Merete Molton, Molden Tor Faksvaag, Kommedal Øyvind, Hovig Eivind, Leegaard Truls Michael, Ambur Ole Herman

Abstract excerpt

Sensitive and specific genotyping of human papillomaviruses (HPVs) is important for population-based surveillance of carcinogenic HPV types and for monitoring vaccine effectiveness. Here we compare HPV genotyping by Next Generation Sequencing (NGS) to an established DNA hybridization method. In DNA isolated from urine, the overall analytical sensitivity of NGS was found to be 22% higher than that of...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 28045981
DOI: 10.1371/journal.pone.0169074
PMCID: PMC5207713

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MGP

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