Article
Mutational analysis of Kex2 recognition sites and a disulfide bond in tannase from Aspergillus oryzae.
Biochemical and biophysical research communications - 22 Jan 2017
Koseki Takuya, Otsuka Motohiro, Mizuno Toshiyuki, Shiono Yoshihito
Abstract excerpt
Aspergillus oryzae tannase (AoTanA), which contains two Kex2 recognition sites at positions Arg311 and Arg316, consists of two subunits that are generated by the cleavage of tannase gene product by the Kex2 protease. Based on the crystal structure of feruloyl esterase from Aspergillus oryzae (AoFaeB), which has been classified as a member of the fungal tannase family, the catalytic triad residues of AoTanA are...
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