Article
A Near-Atomic Structure of the Dark Apoptosome Provides Insight into Assembly and Activation.
Structure (London, England : 1993) - 3 Jan 2017
Cheng Tat Cheung, Akey Ildikó V, Yuan Shujun, Yu Zhiheng, Ludtke Steven J, Akey Christopher W
Abstract excerpt
In Drosophila, the Apaf-1-related killer (Dark) forms an apoptosome that activates procaspases. To investigate function, we have determined a near-atomic structure of Dark double rings using cryo-electron microscopy. We then built a nearly complete model of the apoptosome that includes 7- and 8-blade β-propellers. We find that the preference for dATP during Dark assembly may be governed by Ser325, which is in...
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