Article
Generation and Screening of an Insertion Sequencing-Compatible Mutant Library of Campylobacter jejuni.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2017
Johnson Jeremiah G, DiRita Victor J
Abstract excerpt
The advent of next-generation sequencing technology has enabled experimental approaches to characterize large, complex populations of DNA molecules with high resolution. Included among these are methods to assess populations of transposon insertion libraries for the fitness cost of any particular mutant allele after applying selection to a population. These approaches have proven invaluable for identifying...
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