Article
Generation of human induced pluripotent stem cells in defined, feeder-free conditions.
Stem cell research - 1 Sept 2016
Kyrkou Athena, Stellas Dimitris, Syrrou Marika, Klinakis Apostolos, Fotsis Theodore, Murphy Carol
Abstract excerpt
Herein, we describe a modified protocol for the generation of human induced pluripotent stem cells (hiPS) and expansion under defined, serum free and feeder free conditions. These cells exhibit a high level of plasticity towards various differentiation pathways both in vitro and in vivo. Ultimately, hiPS-derived lines achieved high standards of three dimensional differentiations on biomaterial scaffolds and...
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