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Ratiometric analysis of Acridine Orange staining in the study of acidic organelles and autophagy

15 Nov 2016

Abstract excerpt

Acridine Orange is a cell-permeable green fluorophore that can be protonated and trapped in acidic vesicular organelles (AVOs). Its metachromatic shift to red fluorescence is concentration-dependent and, therefore, Acridine Orange fluoresces red in AVOs, such as autolysosomes. This makes Acridine Orange staining a quick, accessible and reliable method to assess the volume of AVOs, which increases upon autophagy...

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PMID: 27875278
DOI: 10.1242/jcs.195057

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