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Article

A Modified Protocol with Improved Detection Rate for Mis-Matched Donor HLA from Low Quantities of DNA in Urine Samples from Kidney Graft Recipients.

PloS one - 1 Jan 2016

Kwok Janette, Choi Leo C W, Ho Jenny C Y, Chan Gavin S W, Mok Maggie M Y, Lam Man-Fei, Chak Wai-Leung, Cheuk Au, Chau Ka-Foon, Tong Matthew, Chan Kwok-Wah, Chan Tak-Mao

Abstract excerpt

BACKGROUND: Urine from kidney transplant recipient has proven to be a viable source for donor DNA. However, an optimized protocol would be required to determine mis-matched donor HLA specificities in view of the scarcity of DNA obtained in some cases. METHODS: In this study, fresh early morning urine specimens were obtained from 155 kidney transplant recipients with known donor HLA phenotype. DNA was extracted...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

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Identifiers

PMID: 27861530
DOI: 10.1371/journal.pone.0166427
PMCID: PMC5115744

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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