Article
T-DNA integration in plants results from polymerase-θ-mediated DNA repair.
Nature plants - 31 Oct 2016
van Kregten Maartje, de Pater Sylvia, Romeijn Ron, van Schendel Robin, Hooykaas Paul J J, Tijsterman Marcel
Abstract excerpt
Agrobacterium tumefaciens is a pathogenic bacterium, which transforms plants by transferring a discrete segment of its DNA, the T-DNA, to plant cells. The T-DNA then integrates into the plant genome. T-DNA biotechnology is widely exploited in the genetic engineering of model plants and crops. However, the molecular mechanism underlying T-DNA integration remains unknown1. Here we demonstrate that in Arabidopsis...
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