Article
Characterization of the Neisseria meningitidis Helicase RecG.
PloS one - 1 Jan 2016
Beyene Getachew Tesfaye, Balasingham Seetha V, Frye Stephan A, Namouchi Amine, Homberset Håvard, Kalayou Shewit, Riaz Tahira, Tønjum Tone
Abstract excerpt
Neisseria meningitidis (Nm) is a Gram-negative oral commensal that opportunistically can cause septicaemia and/or meningitis. Here, we overexpressed, purified and characterized the Nm DNA repair/recombination helicase RecG (RecGNm) and examined its role during genotoxic stress. RecGNm possessed ATP-dependent DNA binding and unwinding activities in vitro on a variety of DNA model substrates including a Holliday...
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