Article
Tissue Culture as a Source of Replicates in Nonmodel Plants: Variation in Cold Response in Arabidopsis lyrata ssp. petraea.
G3 (Bethesda, Md.) - 7 Dec 2016
Kenta Tanaka, Edwards Jessica E M, Butlin Roger K, Burke Terry, Quick W Paul, Urwin Peter, Davey Matthew P
Abstract excerpt
While genotype-environment interaction is increasingly receiving attention by ecologists and evolutionary biologists, such studies need genetically homogeneous replicates-a challenging hurdle in outcrossing plants. This could be potentially overcome by using tissue culture techniques. However, plants regenerated from tissue culture may show aberrant phenotypes and "somaclonal" variation. Here, we examined...
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