Article
Cryopreservation of Picea sitchensis (sitka spruce) embryogenic suspensor masses.
Cryo letters - 1 Jan 2000
Gale Samantha, John Allan, Benson Erica E
Abstract excerpt
A cryopreservation method comprising sorbitol pre-growth treatment, DMSO cryoprotection and two-step controlled rate cooling has been optimized for differentiated embryogenic suspensor masses (ESM) of Picea sitchensis. The protocol was applied to clonal cultures from five different half-sibling families each represented by five different genotypes and their responses to cryopreservation assessed over 3 years....
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