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Article

High throughput cryopreservation of 140,000 Physcomitrella patens mutants.

Plant biology (Stuttgart, Germany) - 1 Jan 2000

Schulte J, Reski R

Abstract excerpt

A high throughput protocol was established to preserve 140,000 mutants of a moss, Physcomitrella patens, a model plant for functional genomics studies, over liquid nitrogen. Regarding the reliable long-term storage of diverse mutant phenotypes, as well as time and cost effectiveness, each working step was optimized: 1) plant preparation, 2) freezing regime, cryogenic conditions, 3) regrowth after thawing. A...

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PMID: 15045662
DOI: 10.1055/s-2004-817796

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