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Article

A rational approach for cancer stem-like cell isolation and characterization using CD44 and prominin-1(CD133) as selection markers.

Oncotarget - 29 Nov 2016

Lee Yi-Jen, Wu Chang-Cheng, Li Jhy-Wei, Ou Chien-Chih, Hsu Shih-Chung, Tseng Hsiu-Hsueh, Kao Ming-Ching, Liu Jah-Yao

Abstract excerpt

The availability of adequate cancer stem cells or cancer stem-like cell (CSC) is important in cancer study. From ovarian cancer cell lines, SKOV3 and OVCAR3, we induced peritoneal ascites tumors in immunodeficient mice. Among the cells (SKOV3.PX1 and OVCAR3.PX1) from those tumors, we sorted both CD44 and CD133 positive cells (SKOV3.PX1_133+44+, OVCAR3.PX1_133+44+), which manifest the characteristics of...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 27655682
DOI: 10.18632/oncotarget.12100
PMCID: PMC5346656

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