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Article

Stability enhancement of cytochrome c through heme deprotonation and mutations.

Biophysical chemistry - 1 Jan 2009

Sonoyama Takafumi, Hasegawa Jun, Uchiyama Susumu, Nakamura Shota, Kobayashi Yuji, Sambongi Yoshihiro

Abstract excerpt

The chemical denaturation of Pseudomonas aeruginosa cytochrome c(551) variants was examined at pH 5.0 and 3.6. All variants were stabilized at both pHs compared with the wild-type. Remarkably, the variants carrying the F34Y and/or E43Y mutations were more stabilized than those having the F7A/V13M or V78I ones at pH 5.0 compared with at pH 3.6 by ~3.0-4.6 kJ/mol. Structural analyses predicted that the side chains...

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Identifiers

PMID: 18957274
DOI: 10.1016/j.bpc.2008.09.020

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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