Article
Truncation and Mutation of a Transferrin Receptor Aptamer Enhances Binding Affinity.
Nucleic acid therapeutics - 1 Dec 2016
Macdonald Joanna, Houghton Patrick, Xiang Dongxi, Duan Wei, Shigdar Sarah
Abstract excerpt
Aptamers are proving their utility in a number of applications. However, to be easily functionalized, their structure needs to be simplified. Therefore, we sought to truncate a 50-nucleotide aptamer specific to the transferrin receptor to its smallest functional unit using rational engineering of the predicted two-dimensional structure of the longer parent sequence. In addition, mutations were introduced into the...
Topics
- Animals
- Aptamers, Nucleotide
- Base Pairing
- Base Sequence
- Cell Line
- Cell Line, Tumor
- Endocytosis
- Endothelial Cells
- Gene Expression
- Humans
- Kinetics
- Lymphocytes
- Mice
- Mutation
- Nucleic Acid Conformation
- Receptors, Transferrin
- SELEX Aptamer Technique